Multiplex Fluorescent PCR-Amplification Technology
Multiplex Fluorescent PCR-Amplification Technology
Multiplex Fluorescent PCR is an advanced molecular biology technique that combines multiplex PCR (amplification of multiple DNA targets in a single reaction) with fluorescent labeling for simultaneous detection and analysis. This method is widely used in diagnostics, genotyping, pathogen detection, and genetic research due to its high throughput, sensitivity, and specificity.
Applications
Applications
Introduction
Introduction
Pathogen Detection (e.g., respiratory viruses, sexually transmitted infections).
Genetic Testing (e.g., SNP genotyping, cancer mutations).
Food Safety (GMO detection, microbial contamination).
Forensics (STR analysis, human identification).
Advantages
Advantages
High Throughput: Detects multiple targets in one reaction.
Cost-Effective: Reduces reagent use and labor compared to singleplex PCR.
Sensitive & Specific: Fluorescent probes enhance accuracy.
Quantitative: Real-time monitoring allows for DNA quantification.
